The church sent a letter to leaders of the troop this week and referred to the national organization's decision.
"We underscore our grief at the decisions made by the BSA which have led to this point," the letter said.
In May, Boy Scout delegates met in Grapevine, Texas, and by a 61 percent margin of 1,400 voters approved the proposal. The delegation, however, stopped short of allowing gay adults to supervise the youths.
Brian T. FitzGerald, former scoutmaster for Troop 4, said there are about 120 boys in the Boy Scout troop and Cub Scout pack who are being displaced come Dec. 31. "That's unfortunate its affecting us. It's most unfortunate for boys that are in the troop and the boys that are in the cub scout pack. but in a way I guess it's a life lesson" said FitzGerald.
The troop will need to find a new sponsor and a new chartering organization.
FitzGerald says the troop has had a continuous charter at the church for the past 60 years, long before Holy Trinity Presbyterian moved in about four years ago.
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